| Autor: |
Emilio S. Rivera, Andy Weiss, Lukasz G. Migas, Jeffrey A. Freiberg, Katerina V. Djambazova, Elizabeth K. Neumann, Raf Van de Plas, Jeffrey M. Spraggins, Eric P. Skaar, Richard M. Caprioli |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 26, Iss , Pp 36-46 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2667-145X |
| DOI: |
10.1016/j.jmsacl.2022.09.003 |
| Popis: |
Introduction: Although Staphylococcus aureus is the leading cause of biofilm-related infections, the lipidomic distributions within these biofilms is poorly understood. Here, lipidomic mapping of S. aureus biofilm cross-sections was performed to investigate heterogeneity between horizontal biofilm layers. Methods: S. aureus biofilms were grown statically, embedded in a mixture of carboxymethylcellulose/gelatin, and prepared for downstream matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS). Trapped ion mobility spectrometry (TIMS) was also applied prior to mass analysis. Results: Implementation of TIMS led to a ∼ threefold increase in the number of lipid species detected. Washing biofilm samples with ammonium formate (150 mM) increased signal intensity for some bacterial lipids by as much as tenfold, with minimal disruption of the biofilm structure. MALDI TIMS IMS revealed that most lipids localize primarily to a single biofilm layer, and species from the same lipid class such as cardiolipins CL(57:0) – CL(66:0) display starkly different localizations, exhibiting between 1.5 and 6.3-fold intensity differences between layers (n = 3, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect