| Autor: |
Kit Neikirk, Edgar-Garza Lopez, Andrea G. Marshall, Ahmad Alghanem, Evan Krystofiak, Bartosz Kula, Nathan Smith, Jianqiang Shao, Prasanna Katti, Antentor Hinton, Jr. |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
European Journal of Cell Biology, Vol 102, Iss 4, Pp 151365- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
0171-9335 |
| DOI: |
10.1016/j.ejcb.2023.151365 |
| Popis: |
This review provides an overview of the current methods for quantifying mitochondrial ultrastructure, including cristae morphology, mitochondrial contact sites, and recycling machinery and a guide to utilizing electron microscopy to effectively measure these organelles. Quantitative analysis of mitochondrial ultrastructure is essential for understanding mitochondrial biology and developing therapeutic strategies for mitochondrial-related diseases. Techniques such as transmission electron microscopy (TEM) and serial block face-scanning electron microscopy, as well as how they can be combined with other techniques including confocal microscopy, super-resolution microscopy, and correlative light and electron microscopy are discussed. Beyond their limitations and challenges, we also offer specific magnifications that may be best suited for TEM analysis of mitochondrial, endoplasmic reticulum, and recycling machinery. Finally, perspectives on future quantification methods are offered. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect