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IntroductionThe maximal lactate steady state (MLSS) is defined as the highest workload that can be maintained without blood lactate accumulation over time. The power output at MLSS (PMLSS) is regularly implemented to define training zones, quantify training progress, or predict race performance. The gold standard methodology for MLSS determination requires two to five trials of constant-load exercise, which limits the practical application in training. The INSCYD software can calculate the PMLSS (PMLSSINSCYD) based on physiological data that can be obtained during a ∼1 h laboratory visit. However, to the best of our knowledge, the validity of the most recent software version has not yet been investigated. This study aimed to assess the validity of the software's calculations on PMLSS in cycling.MethodsThe data for this study were retrieved from two published scientific sources. Thirty-one cyclists (19 males, 12 females) performed a 15 s sprint to estimate the VLamax, a ramp test for the V˙O2max assessment, and two to five constant-load tests to determine the PMLSS. The INSCYD software was used to calculate the PMLSS based on the V˙O2max, VLamax, sex, body mass, and body composition.ResultsThe PMLSSINSCYD was higher than the PMLSS in the entire sample (mean difference: 4.6 W, p |