High-quality genome assembly of a C. crossoptilon and related functional and genetics data resources

Autor: Siwen Wu, Kun Wang, Tengfei Dou, Sisi Yuan, Dong-Dong Wu, Changrong Ge, Junjing Jia, Zhengchang Su
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Scientific Data, Vol 11, Iss 1, Pp 1-6 (2024)
Druh dokumentu: article
ISSN: 2052-4463
DOI: 10.1038/s41597-024-03087-5
Popis: Abstract There are four species in the Crossoptilon genus inhibiting at from very low to very high altitudes across China, and they are in varying levels of danger of extinction. To better understand the genetic basis of adaptation to high altitudes and genetic changes due to bottleneck, we assembled the genome (~1.02 Gb) of a white eared pheasant (WT) (Crossoptilon crossoptilon) inhibiting at high altitudes (3,000~7,000 m) in northwest of Yunnan province, China, using a combination of Illumina short reads, PacBio long reads and Hi-C reads, with a contig N50 of 19.63 Mb and only six gaps. To further provide resources for gene annotation as well as functional and population genetics analyses, we sequenced transcriptomes of 20 major tissues of the WT individual and re-sequenced another 10 WT individuals and a blue eared pheasant (Crossoptilon auritum) individual inhabiting at intermediate altitudes (1,500~3,000 m). Our assembled WT genome, transcriptome data, and DNA sequencing data can be valuable resources for studying the biology, evolution and developing conservation strategies of these endangered species.
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