Assessment of potential pathogenicity among clinical strains of Pseudomonas aeruginosa using bioluminescent technique
| Autor: | M. V. Kuznetsova, I. L. Maslennikova, T. I. Karpunina, N. V. Nikolayeva |
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| Jazyk: | English<br />Russian |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Бюллетень сибирской медицины, Vol 11, Iss 4, Pp 44-48 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1682-0363 1819-3684 |
| DOI: | 10.20538/1682-0363-2012-4-44-48 |
| Popis: | The aim: to study the influence of P. aeruginosa exometabolites on E. coli lux+ bioluminescence and experimentally prove the expediency of use of a bioluminescent method for an estimation of their potential pathogenicity. Objectives and Methods. Exometabolites of P. aeruginosa (n = 27) nosocomial strains were used. Index of potential pathogenicity (IPP) was calculated with the formula: IPP = (Ik — Io)/Ik × 100%, where Ik — intensity of Escherichia coli lux+ bioluminescence in control test (LB), Io — intensity of a bioluminescence in experimental test (exometabolites). Results. Protocol of analysis implementation and exposure duration has been determined. According to IPP, P. aeruginosa strains were subdivided into 3 groups: strains with low degree of pathogenicity, moderate and highly pathogenic. The group of IPP ³ 70% was characterized by reliably high correlation coefficient relatively to pyocyanin production, film-forming ability. This indicates the possibility of bioluminescent method application as screening or additional method of the assessment of P. aeruginosa isolate aggressiveness, and in purulent-septic infections (PSI) in particular. |
| Databáze: | Directory of Open Access Journals |
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