Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

Autor: Tuncel Gulten, Ergoren Mahmut Cerkez, Baddal Buket, Tulay Pinar, Arikan Ayse, Guler Emrah, Ozverel Cenk Serhan, Kaya Suer H., Sayan Murat, Sanlidag Tamer
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: The EuroBiotech Journal, Vol 5, Iss s1, Pp 26-31 (2021)
Druh dokumentu: article
ISSN: 2564-615X
DOI: 10.2478/ebtj-2021-0018
Popis: A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2. This study investigated the compatibility of two different RT-qPCR methodologies Diagnovital® and Bio-Speedy® by re-analyzing the previously confirmed positive samples. A total of 43 previously confirmed positive samples were re-analyzed by two different commercially available SARS-CoV-2 RT-qPCR kits. Only 23.5% of positive samples detected by Diagnovital® RT-qPCR kit were detected by Bio-Speedy® detection kit.
Databáze: Directory of Open Access Journals