Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification

Autor: Zhichen Xu, Dongjuan Chen, Tao Li, Jiayu Yan, Jiang Zhu, Ting He, Rui Hu, Ying Li, Yunhuang Yang, Maili Liu
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-022-34086-y
Popis: Fast, low-cost and multiplexed nucleic acid detection is challenging. Here the authors report a strategy that couples microfluidic space coding, CRISPRCas12a, and multiplex RPA for the rapid detection of up to 30 targets with only one fluorescent probe.
Databáze: Directory of Open Access Journals