Evaluating the potential of autologous and allogeneic mice serums in comparison with bovine serum and albumin on oocyte maturation and fertilization in NMRI mice
| Autor: | Shahsavand-Inanloo O, Mohseni-Kouchesfahani H, Parivar K, Amini E, Hadi M |
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| Jazyk: | perština |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Fiyz̤, Vol 24, Iss 5, Pp 481-490 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1029-7855 2008-9821 |
| Popis: | Background: Sera are a multifaceted mixture of growth factors, proteins, and etc. which can influence oocyte maturation and fertilization. The aim of this survey is assessing the effect of different sera on in vitro maturation and fertilization in NMRI mice. Materials and Methods: In this experimental study, maturation of oocytes was evaluated in α-MEM medium treated with 0 to 20 allogenic and autologous mice serums, Fetal Bovine Serum (FBS) and Bovine Serum Albumin (BSA) after 14-18h incubation period. Then MII oocytes were fertilized with capacitated sperms that percentages of 0 to 20 and investigated fertilization ratio. Results: In vitro maturation and in vitro fertilization level elevated significantly by adding serum to the culture medium (P |
| Databáze: | Directory of Open Access Journals |
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