Autor: |
Sho Yoshimatsu, Rei Murakami, Tsukika Sato, Tsubasa Saeki, Masafumi Yamamoto, Erika Sasaki, Toshiaki Noce, Hideyuki Okano |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Stem Cell Research, Vol 53, Iss , Pp 102308- (2021) |
Druh dokumentu: |
article |
ISSN: |
1873-5061 |
DOI: |
10.1016/j.scr.2021.102308 |
Popis: |
POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA). |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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