| Autor: |
Sherin Bakhashab, Ghalya H. Banafea, Farid Ahmed, Haneen Alsehli, Huda F. AlShaibi, Nadia Bagatian, Ohoud Subhi, Kalamegam Gauthaman, Mahmood Rasool, Hans-Juergen Schulten, Peter Natesan Pushparaj |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Experimental and Molecular Pathology, Vol 132, Iss , Pp 104867- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1096-0945 |
| DOI: |
10.1016/j.yexmp.2023.104867 |
| Popis: |
Mast cells (MCs) are tissue-resident innate immune cells that express the high-affinity receptor for immunoglobulin E and are responsible for host defense and an array of diseases related to immune system. We aimed in this study to characterize the pathways and gene signatures of human cord blood-derived MCs (hCBMCs) in comparison to cells originating from CD34− progenitors using next-generation knowledge discovery methods. CD34+ cells were isolated from human umbilical cord blood using magnetic activated cell sorting and differentiated into MCs with rhIL-6 and rhSCF supplementation for 6–8 weeks. The purity of hCBMCs was analyzed by flow cytometry exhibiting the surface markers CD117+CD34−CD45−CD23−FcεR1αdim. Total RNA from hCBMCs and CD34− cells were isolated and hybridized using microarray. Differentially expressed genes were analyzed using iPathway Guide and Pre-Ranked Gene Set Enrichment Analysis. Next-generation knowledge discovery platforms revealed MC-specific gene signatures and molecular pathways enriched in hCBMCs and pertain the immunological response repertoire. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect