| Autor: |
Kseniya Tishchenko, Marina Muratova, Dmitry Volkov, Vera Filichkina, Dmitry Nedosekin, Vladimir Zharov, Mikhail Proskurnin |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Arabian Journal of Chemistry, Vol 10, Iss 6, Pp 781-791 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1878-5352 |
| DOI: |
10.1016/j.arabjc.2016.01.009 |
| Popis: |
Accurate measurements of absorptivities and quantum yields of biochemical species under various conditions are an important task of applied photonics and analytical chemistry. In this work, we provide a comparison of the capabilities of thermal-lens spectrometry to measure these parameters of various samples. Measurements of relevant model substances, biologically active substituted 2-thiohydantoins and their complexes of copper(I,II) and heme proteins (forms of hemoglobin and cytochrome c), showed negligible differences in apparent molar absorptivities for thermal-lens spectrometry and optical-absorption (spectrophotometric) data. The values for tabletop and microscale thermal-lens measurements under batch conditions differ insignificantly. The precision of measurements of molar absorptivities by thermal-lens spectrometry is no less than in the case of spectrophotometry or the precision is even higher in the cases of low absorptivities. For cardiolipin–cytochrome c-NO complex, the difference between absorptivity values calculated from thermal-lens data and acquired by spectrophotometry is significant due to complex photodegradation. The quantum yield of its photolysis reaction calculated from optical absorption and thermal-lens data altogether at two wavelengths for 0.4–360 s of the reaction, 0.46 ± 0.04, was estimated. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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