Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice

Autor: Adrian Rosenberg, Daiki Fujimura MD, Ryuhei Okada MD, PhD, Aki Furusawa PhD, Fuyuki Inagaki MD, PhD, Hiroaki Wakiyama MD, PhD, Takuya Kato MD, PhD, Peter L. Choyke MD, Hisataka Kobayashi MD, PhD
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Molecular Imaging, Vol 19 (2020)
Druh dokumentu: article
ISSN: 1536-0121
15360121
DOI: 10.1177/1536012120934965
Popis: Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment. Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour. Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg −1 and 0.3 mg kg −1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly. Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.
Databáze: Directory of Open Access Journals
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