Features of 'All LNA' Duplexes Showing a New Type of Nucleic Acid Geometry
| Autor: | Charlotte Förster, André Eichert, Dominik Oberthür, Christian Betzel, Reinhard Geßner, Andreas Nitsche, Jens P. Fürste |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Journal of Nucleic Acids, Vol 2012 (2012) |
| Druh dokumentu: | article |
| ISSN: | 2090-0201 2090-021X |
| DOI: | 10.1155/2012/156035 |
| Popis: | “Locked nucleic acids” (LNAs) belong to the backbone-modified nucleic acid family. The 2′-O,4′-C-methylene-β-D-ribofuranose nucleotides are used for single or multiple substitutions in RNA molecules and thereby introduce enhanced bio- and thermostability. This renders LNAs powerful tools for diagnostic and therapeutic applications. RNA molecules maintain the overall canonical A-type conformation upon substitution of single or multiple residues/nucleotides by LNA monomers. The structures of “all” LNA homoduplexes, however, exhibit significant differences in their overall geometry, in particular a decreased twist, roll and propeller twist. This results in a widening of the major groove, a decrease in helical winding, and an enlarged helical pitch. Therefore, the LNA duplex structure can no longer be described as a canonical A-type RNA geometry but can rather be brought into proximity to other backbone-modified nucleic acids, like glycol nucleic acids or peptide nucleic acids. LNA-modified nucleic acids provide thus structural and functional features that may be successfully exploited for future application in biotechnology and drug discovery. |
| Databáze: | Directory of Open Access Journals |
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