Preparation of Bacillus subtilis cell samples and generation of an SDS-PAGE
| Autor: | Thu TM Dao, Dat D Truong, Lan NH Duong, Nhi NY Nguyen, Hoang D Nguyen |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 74, Iss 3, Pp 123-129 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2022-0083 |
| Popis: | Bacillus subtilis is a critical host for producing recombinant proteins. However, the SDS-PAGE process, including the sample preparation steps, varies among B. subtilis-related studies, making it impossible to compare findings. Hence, this paper provides a simple guide to culture and collect B. subtilis cells through an OD600 measurement and a protocol for SDS-PAGE. These techniques were applied to check the expression of a BgaB, a reporter protein and LukF-PV, a potential vaccine candidate against S. aureus, in the cytoplasm of B. subtilis under the control of a strong promoter, Pgrac212. This protocol could be helpful for scientists in preparing samples and generating an SDS-PAGE experiment, as well as favoring the unification of research about protein expression in B. subtilis. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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