Multifunctional Modification of Biologically Active Substances
| Autor: | Moskvicheva Yana Borisovna, Petrovskiy Stanislav Viktorovich, Ginak Anatoliy Iosifovich |
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| Jazyk: | ruština |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Vestnik Volgogradskogo Gosudarstvennogo Universiteta. Seriâ 11. Estestvennye Nauki, Iss 1, Pp 13-19 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2306-4153 2409-2762 |
| DOI: | 10.15688/jvolsu11.2014.1.2 |
| Popis: | The article studies the interracting of oxidized cyclic cyclodextrin dextrans (CD) with the protein - bacterial streptokinase. The oxidation was carried out with potassium periodate; free aldehyde groups were formed in CD molecule during this process. Excess of potassium periodate was removed on a column using ion – exchange resin (anionite APA with acetate ion). Reaction between streptokinase and oxidized cyclic dextran was monitored by gel-permation chromatography (sephadex G-200). The mass of complex of streptokinase with exciding cyclodextrin goes up to 65 kDa (the ratio by moles is 1-3). One streptokinase macromolecule is bound to 14 molecules of CD. It was shown that the chemical reaction between streptokinase and cyclodextrin proceeded in a way which is similar to its reaction with oxidized linear dextrans. It was found that protein-bound CD retained the ability to absorb small molecules like adenosine in its inner shell. The complex of streptokinase with CD absorbs the molecule of adenosine and forms a new complex with adenosine intercalated in cyclodextrin part of the new complex. |
| Databáze: | Directory of Open Access Journals |
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