| Autor: |
Elżbieta Malarczyk, Janina Kochmańska-Rdest, Marzanna Paździoch-Czochra |
| Jazyk: |
angličtina |
| Rok vydání: |
2004 |
| Předmět: |
|
| Zdroj: |
Dose-Response, Vol 2 (2004) |
| Druh dokumentu: |
article |
| ISSN: |
1559-3258 |
| DOI: |
10.1080/15401420490464466 |
| Popis: |
Changes in the activity of horseradish peroxidase resulting from an addition of ethanol water dilutions of 19 phenolic compounds were observed. For each compound, the enzyme activity was plotted against the degree of dilution expressed as n = − log 100 (mol/L) in the range 0 ≤ n ≥ 20. All the curves showed sinusoidal activity, more or less regular, with two to four peaks on average. Each analyzed compound had a characteristic sinusoidal shape, which was constant for samples of peroxidase from various commercial firms. This was clearly visible after function fitting to experimental results based on the Marquadt-Levenberg algorithm using the least-squares method. Among the 19 phenolics, the highest amplitudes were observed for phenol and iso- and vanillate acids and aldehydes. The specific character of each of the analyzed curves offers a possibility of choosing proper dilutions of phenolic compound for activating or inhibiting of peroxidase activity. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
