Evaluation of ERK activity on Ischemic Tolerance-induced by Preconditioning with Intermittent Normobaric Hyperoxia in the Rat Model of Stroke
| Autor: | Firoozeh Alavian, Sohrab Hajizadeh, Mohammad Javan, Roham Mazloom |
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| Jazyk: | perština |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk, Vol 20, Iss 6, Pp 41-53 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1735-5338 2008-644X |
| Popis: | Abstract Background: Recent studies indicate that hyperoxia has a significant therapeutic effect in the acute ischemic injury. The role of intracellular kinases, including ERK, has been posed in the phenomenon of ischemic tolerance. In the present study, the effect of intermittent normobaric hyperoxia on the activity of ERK in the stroke model was studied Material and Methods: This is an experimental study. Animals include 4 groups (sham, hyperoxia–sham, stroke and hyperoxia–stroke); each group consisted of 6 male Wistar rats in the weight range of 250 to 350 grams. Hyperoxia groups were exposed to 95% inspired oxygen for 4 h/day and 6 consecutive days. Oxygen concentration in the control groups was 21% (normoxia, room air). After 24h, the stroke group animals were subjected to 60 min of right middle cerebral artery occlusion (MCAO). After 24h reperfusion, neurological deficit scores (NDS) and ERK activity were assessed. Results: 5 hours after MCAO, stroke groups showed a significant increase in ERK activity in the cortex (p |
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