| Autor: |
Dimitrios Fanourakis, Nikolaos Nikoloudakis, Konstantinos Paschalidis, Miltiadis V. Christopoulos, Eleni Goumenaki, Eleni Tsantili, Costas Delis, Georgios Tsaniklidis |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Agriculture, Vol 12, Iss 6, p 778 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2077-0472 |
| DOI: |
10.3390/agriculture12060778 |
| Popis: |
Beta-galactosidases (β-GALs) hold a key role in both fruit softening and the increase of total soluble solids during maturation. Despite determining both quality and potential postharvest longevity, β-GAL activity during ripening, with a special focus on the postharvest period, has not been adequately addressed in a spatial and temporal manner. This study focused on the regulation of gene expression in relation to the total β-GAL enzyme activity during the ripening of tomato fruit attached on the plant, as well as harvested fruit ripened for 5 d at 4, 10, or 25 °C. The transcription of genes coding for β-GAL isoenzymes was significantly affected by both the fruit maturation stage (unripe vs. red ripe) and postharvest storage temperature. Cold stressed tomatoes (4 °C) exhibited a remarkably higher transcription of most β-GAL genes compared to on-plant red ripe fruit and to fruit exposed to either 10 or 25 °C, indicating a low temperature response. However, enzymatic activity and water-soluble pectin content increased with elevated temperature exposure, peaking in fruit stored at 25 °C. β-GAL activity was present in the pericarp, while it was less detected in locular parenchyma. These findings highlight the dual role of β-GAL not only in maturation, but also in the metabolism during postharvest homeostasis and cold acclimation of tomato fruit. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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