Application of monocyte monolayer assay on hemolytic disease of fetus and newborn caused by IgG anti-M
| Autor: | Chunyan MO, Shuangshuang JIA, Siying ZHU, Yanli JI, Yuan SHAO, Zhijian LIAO, Guangping LUO, Ling WEI |
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| Jazyk: | čínština |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Zhongguo shuxue zazhi, Vol 37, Iss 6, Pp 643-647 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1004-549X 1004-549x |
| DOI: | 10.13303/j.cjbt.issn.1004-549x.2024.06.006&lang=en |
| Popis: | Objective To elucidate the prediction ability of monocyte monolayer assay(MMA) used in hemolytic disease of fetus and newborn(HDFN) caused by IgG anti-M. Methods Plasma from eight pregnant women containing IgG anti-M were collected, and were divided into two groups(4 cases with HDFN, with severe clinical symptoms such as fetal hydrops, and 4 cases without HDFN) according to the clinical outcomes. M antigen positive cells were sensitized with dithiothreitol(DTT) treated plasma from eight pregnant women respectively. MMA was performed by coincubation with monocytes and sensitized M cells, along with negative and positive control set up. T-test was conducted to compare the difference in phagocytic efficiency between two groups. Results The phagocytic efficiency in group with HDFN were 15.37%, 13.05%, 9.17% and 24.50% respectively, with the mean value of 15.52%, while the group without HDFN were 8.74%, 11.07%, 5.12% and 6.23% respectively, with the mean value of 7.79%.There was no significant difference in phagocytic efficiency between two groups(P>0.05). The mean values of both groups were not significantly different from the negative control(P>0.05), but both were significantly lower than positive control(P |
| Databáze: | Directory of Open Access Journals |
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