| Autor: |
José David Sánchez-Martínez, Gerardo Alvarez-Rivera, Rocio Gallego, Mariane Bittencourt Fagundes, Alberto Valdés, Jose A. Mendiola, Elena Ibañez, Alejandro Cifuentes |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Food Chemistry: X, Vol 13, Iss , Pp 100242- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2590-1575 |
| DOI: |
10.1016/j.fochx.2022.100242 |
| Popis: |
Pressurized liquid extraction (PLE) conditions were optimized to improve the recovery of orange (Citrus sinensis) by-products terpenoids. The neuroprotective potential of the PLE extracts were tested against a set of in-vitro assay (antioxidant (ABTS), reactive oxygen/nitrogen species (ROS/RNS)) as well as enzymatic tests (acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and lipoxygenase (LOX)). Gas chromatography coupled to high-resolution mass spectrometry (GC-q-TOF-MS) analysis revealed a higher enrichment in mono- and sesquiterpenoids of the PLE extracts with the highest neuroprotection capacity. In-silico molecular docking analysis showed the specific interaction of representative terpenoids with enzymes active sites. The results demonstrate that the selected extract at 100 °C and 30 minutes possesses high antioxidant (ABTSIC50 = 13.5 μg mL−1; ROSIC50 = 4.4 μg mL−1), anti-cholinesterase (AChEIC50 = 137.1 vg L−1; BChEIC50 = 147.0 μg mL−1) and anti-inflammatory properties (against IL-6 and LOXIC50 = 76.1 μg mL−1), with low cytotoxicity and protection against L-glutamic acid in cell models. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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