| Autor: |
Sang Wha Kim, Won Hee Hong, Se Jin Han, Jun Kwon, Heejun Ko, Sung Bin Lee, Sib Sankar Giri, Sang Guen Kim, Byung Yeop Kim, Goo Jang, Byeong Chun Lee, Dong Wan Kim, Se Chang Park |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Frontiers in Marine Science, Vol 7 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2296-7745 |
| DOI: |
10.3389/fmars.2020.571741 |
| Popis: |
Shark populations are constantly decreasing owing to environmental destruction and overfishing; thus, sharks are now at a risk of extinction, with 27.9% of shark species classified as endangered on the International Union for Conservation of Nature’s Red List. Sharks are apex predators and a keystone species in balancing the marine food chain; their extinction will create an imbalance of the entire marine ecosystem. Assisted reproductive technology is the last resort for protecting animals facing severe extinction. Here, as a proactive effort toward building a hormone-induced artificial insemination protocol for endangered wild sharks, we identified the possibility of germ cell maturation by administration of Ovaprim®, a commercially produced synthetic salmon gonadotropin-releasing hormone, and calculated its optimum dosage and injection timing. The experiment was conducted on two shark species—Triakis scyllium and Triaenodon obesus. We found that intramuscular injections of 0.2 mL/kg of Ovaprim® for male T. scyllium and T. obesus, 0.2 mL/kg + 0.5 mL/kg at a 24 h interval for female T. scyllium, and 0.2 mL/kg + 0.2 mL/kg or 0.2 mL/kg + 0.3 mL/kg at a 24 h interval for female T. obesus were optimal dose protocols. These doses effectively induced the maturation and ovulation of oocytes and the release of semen. Our results confirm that Ovaprim® is a suitable tool for shark hormone-induced artificial insemination and indicate that this method may enable the conservation of the endangered shark species. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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