| Autor: |
Jung Soo Kim, Jaeho Choi, Chang Seok Ki, Ki Hoon Lee |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2296-4185 |
| DOI: |
10.3389/fbioe.2021.653509 |
| Popis: |
Since articular cartilage does not regenerate itself, researches are underway to heal damaged articular cartilage by applying biomaterials such as a hydrogel. In this study, we have constructed a dual-layer composite hydrogel mimicking the layered structure of articular cartilage. The top layer consists of a high-density PEG hydrogel prepared with 8-arm PEG and PEG diacrylate using thiol-norbornene photo-click chemistry. The compressive modulus of the top layer was 700.1 kPa. The bottom layer consists of a low-density PEG hydrogel reinforced with a 3D silk fiber construct. The low-density PEG hydrogel was prepared with 4-arm PEG using the same cross-linking chemistry, and the compressive modulus was 13.2 kPa. Silk fiber was chosen based on the strong interfacial bonding with the low-density PEG hydrogel. The 3D silk fiber construct was fabricated by moving the silk fiber around the piles using a pile frame, and the compressive modulus of the 3D silk fiber construct was 567 kPa. The two layers were joined through a covalent bond which endowed sufficient stability against repeated torsions. The final 3D silk fiber construct embedded dual-layer PEG hydrogel had a compressive modulus of 744 kPa. Chondrogenic markers confirmed the chondrogenic differentiation of human mesenchymal stem cells encapsulated in the bottom layer. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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