A 4-bp deletion in the 5’UTR of TaAFP-B is associated with seed dormancy in common wheat (Triticum aestivum L.)

Autor: Yumei Feng, Meng Liu, Zeng Wang, Xianlin Zhao, Bing Han, Yanping Xing, Maoyan Wang, Yan Yang
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: BMC Plant Biology, Vol 19, Iss 1, Pp 1-10 (2019)
Druh dokumentu: article
ISSN: 1471-2229
DOI: 10.1186/s12870-019-1950-4
Popis: Abstract Background AFP is a negative regulator of ABA signaling that promotes ABI5 protein degradation and weakens regulation of ABA signaling by targeting upstream genes of ABI5, and TaABI5 gene was seed-specific, and accumulated during wheat grain maturation and dormancy acquisition, which played an important role in seed dormancy; TaAFP has a conserved domain with AFP, so TaAFP may also play an important role in seed dormancy in wheat. Results Two allelic variants of TaAFP were identified on chromosome 2BS in common wheat, and designated as TaAFP-B1a and TaAFP-B1b. Sequence analysis showed a 4-bp deletion in the 5’UTR region of TaAFP-B1b compared with TaAFP-B1a. Based on the 4-bp deletion, a co-dominant functional marker of TaAFP-B was developed and designated as AFPB. The genotype generating a 203-bp fragment (TaAFP-B1b) was more resistant to pre-harvest sprouting than the genotype producing a 207-bp fragment (TaAFP-B1a) in a test of 91 white-grained Chinese wheat cultivars and advanced lines. The average germination index(GI) values of TaAFP-B1a and that of TaAFP-B1b were 45.18 and 30.72%, respectively, indicating a significant difference (P
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