Association of the Toxigenic Dinoflagellate Alexandrium ostenfeldii With Spirolide Accumulation in Cultured Mussels (Mytilus galloprovincialis) From Northwest Mexico

Autor: Patricia Paredes-Banda, Ernesto García-Mendoza, Elizabeth Ponce-Rivas, Juan Blanco, Antonio Almazán-Becerril, Clara Galindo-Sánchez, Allan Cembella
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Frontiers in Marine Science, Vol 5 (2018)
Druh dokumentu: article
ISSN: 2296-7745
DOI: 10.3389/fmars.2018.00491
Popis: Spirolides are polyether cyclic imines considered as “fast acting toxins.” Long-term human health consequences of spirolide ingestion are uncertain, and hence regulatory limits for human consumption have not been established. Nevertheless, monitoring these toxins in shellfish is essential because they can interfere with detection by mouse bioassay of lipophilic regulated toxins. Todos Santos Bay (TSB), in the northwest of the Baja California Peninsula, is an important shellfish cultivation and fish-farming area in Mexico. The toxin analog 13-desmethyl spirolide C has been reported in cultivated mussels (Mytilus galloprovincialis) from TSB, but the causative species associated with accumulation of this toxin has not been previously identified. We assessed the occurrence of Alexandrium ostenfeldii, the unique known producer of spirolides, by inverted light microscopy and by PCR with species-specific oligonucleotides designed for the ITS and 18S rDNA. We determined the presence and abundance of this species at the surface and at the thermocline from samples collected over two annual sampling periods (2013–2014 and 2016–2017). During the 2013–2014 period, A. ostenfeldii was found in 50% of the samples analyzed by light microscopy. The highest cell abundance (about 3.6 × 103 cells L-1) occurred in October 2013. During 2016–2017 the dinoflagellate was present in low cell abundances (
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