METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS
| Autor: | M. I. Doronin, A. M. Timina, D. A. Lozovoy, V. A. Starikov, D. V. Mikhalishin, N. N. Medvedeva, A. V. Borisov |
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| Jazyk: | English<br />Russian |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Ветеринария сегодня, Vol 0, Iss 2, Pp 26-30 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2304-196X 2658-6959 |
| DOI: | 10.29326/2304-196X-2018-2-25-26-30 |
| Popis: | Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. Negative regression model of С146S = (30.269 – Ct )/4.155 type allowing determination of FMDV 146S particle concentrations based on the amplification threshold cycle values (Ct ) is proposed. It has been experimentally proven that the quantity of the 146S component determined by the real-time RT-PCR method using developed regression model and contained in the inoculation dose of FMD vaccine confers protection to the vaccinated animals against generalized FMD of A, O, Asia-1 types. rtRT-PCR method is highly sensitive and allows rapid and highly reliable estimation of the 146S antigen concentration in FMD vaccine. The method for 146S particle quantity determination by real-time RT-PCR using the regression model is reliable and demonstrates high correlation (95.5–99.0%) with the complement fixation test results. |
| Databáze: | Directory of Open Access Journals |
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