An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m

Autor:	Tania Santos Giani, Severo de Paoli, Giuseppe Antonio Presta, Adalgisa Ieda Maiworm, Sebastião David Santos-Filho, José Brandão-Neto, Aldo da Cunha Medeiros, Adenilson de Souza da Fonseca, Mario Bernardo-Filho
Jazyk:	angličtina
Rok vydání:	2007
Předmět:	Technetium-99m Red blood cell Oxidant agent Radiobiocomplex Plasma proteins Buzhong Yi Qi Wan Biotechnology TP248.13-248.65
Zdroj:	Brazilian Archives of Biology and Technology, Vol 50, Iss spe, Pp 111-116 (2007)
Druh dokumentu:	article
ISSN:	1516-8913 1678-4324
DOI:	10.1590/S1516-89132007000600013
Popis:	Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated. Buzhong extract at the highest concentrations used altered significantly (pBuzhong Yi Qi Wan (Buzhong) é uma fórmula utilizada na Medicina Tradicional Chinesa para tratamento de distúrbios nos sistemas digestório e circulatório. Constituintes sangüíneos marcados com tecnécio-99m (99mTc) são usados na medicina nuclear. O objetivo deste estudo foi investigar os efeitos do extrato de Buzhong na marcação de constituintes sangüíneos com 99mTc. Amostras de sangue de ratos Wistar foram incubadas com diferentes concentrações do extrato de Buzhong e a marcação de constituintes sangüíneos com 99mTc foi realizado. Plasma e células sangüíneas foram separados, frações solúveis e insolúveis do plasma e das células sangüíneas foram isoladas. A radioatividade nos constituintes sangüíneos foi contada e as porcentagens de radioatividade incorporada (%ATI), determinada. Extrato de Buzhong nas maiores concentrações utilizadas altera significativamente (p
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/c9227b52c5494da7ab1554f19d7ed5ff Zobrazit plný text záznamu View record in DOAJ