Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos
| Autor: | Gaëlle Saade, Eva Bogaerts, Sophie Chiavassa, PhD, Guillaume Blain, Grégory Delpon, PhD, Manon Evin, Youssef Ghannam, Ferid Haddad, PhD, Karin Haustermans, MD, PhD, Charbel Koumeir, PhD, Ellina Macaeva, PhD, Lydia Maigne, PhD, Quentin Mouchard, PhD, Noël Servagent, PhD, Edmond Sterpin, PhD, Stéphane Supiot, MD, PhD, Vincent Potiron, PhD |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Advances in Radiation Oncology, Vol 8, Iss 2, Pp 101124- (2023) |
| Druh dokumentu: | article |
| ISSN: | 2452-1094 17656214 |
| DOI: | 10.1016/j.adro.2022.101124 |
| Popis: | Purpose: Recently, ultrahigh-dose-rate radiation therapy (UHDR-RT) has emerged as a promising strategy to increase the benefit/risk ratio of external RT. Extensive work is on the way to characterize the physical and biological parameters that control the so-called “Flash” effect. However, this healthy/tumor differential effect is observable in in vivo models, which thereby drastically limits the amount of work that is achievable in a timely manner. Methods and Materials: In this study, zebrafish embryos were used to compare the effect of UHDR irradiation (8-9 kGy/s) to conventional RT dose rate (0.2 Gy/s) with a 68 MeV proton beam. Viability, body length, spine curvature, and pericardial edema were measured 4 days postirradiation. Results: We show that body length is significantly greater after UHDR-RT compared with conventional RT by 180 µm at 30 Gy and 90 µm at 40 Gy, while pericardial edema is only reduced at 30 Gy. No differences were obtained in terms of survival or spine curvature. Conclusions: Zebrafish embryo length appears as a robust endpoint, and we anticipate that this model will substantially fasten the study of UHDR proton-beam parameters necessary for “Flash.” |
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