Nanodiamonds in Biotechnology: Application for Purification of Proteins and Test-Systems of Indication

Autor:	Nikita O. Ronzhin, Konstantin A. Kharin, Alexey P. Puzyr, Vladimir S. Bondar
Jazyk:	English<br />Russian
Rok vydání:	2010
Předmět:	modified detonation nanodiamonds recombinant luciferase protein purification biotechnology bioluminescence test-system Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
Zdroj:	Журнал Сибирского федерального университета: Серия Биология, Vol 3, Iss 4, Pp 418-433 (2010)
Druh dokumentu:	article
ISSN:	1997-1389 2313-5530
Popis:	This paper discusses the perspectives of applications of the detonation nanodiamonds in biotechnology. An effective separation of proteins from complex protein mixtures by using of nanodiamonds is shown on the example of purification of recombinant luciferase from the extracts of E. coli bacterial cells: the process requires minimum equipment and time; one technological cycle yields 40-45 % of desired product with high-quality (according to the SDS-PAGE). The application of nanodiamonds for design of indicating systems is demonstrated on the example of a biosensor system based on the nanodiamondluciferase complex and inert polymeric matrix.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/91debe81a25a4b48a6ab5904c04124f4 Zobrazit plný text záznamu View record in DOAJ