| Autor: |
O. Tolga Gül, Kaitlin M. Pugliese, Yongki Choi, Patrick C. Sims, Deng Pan, Arith J. Rajapakse, Gregory A. Weiss, Philip G. Collins |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Biosensors, Vol 6, Iss 3, p 29 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2079-6374 |
| DOI: |
10.3390/bios6030029 |
| Popis: |
As biosensing devices shrink smaller and smaller, they approach a scale in which single molecule electronic sensing becomes possible. Here, we review the operation of single-enzyme transistors made using single-walled carbon nanotubes. These novel hybrid devices transduce the motions and catalytic activity of a single protein into an electronic signal for real-time monitoring of the protein’s activity. Analysis of these electronic signals reveals new insights into enzyme function and proves the electronic technique to be complementary to other single-molecule methods based on fluorescence. As one example of the nanocircuit technique, we have studied the Klenow Fragment (KF) of DNA polymerase I as it catalytically processes single-stranded DNA templates. The fidelity of DNA polymerases makes them a key component in many DNA sequencing techniques, and here we demonstrate that KF nanocircuits readily resolve DNA polymerization with single-base sensitivity. Consequently, template lengths can be directly counted from electronic recordings of KF’s base-by-base activity. After measuring as few as 20 copies, the template length can be determined with |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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