| Autor: |
Shi-Ke Wang, Hua-Fei Su, Yu-Chao Gu, Shu-Ling Lin, Jia-Heng Tan, Zhi-Shu Huang, Tian-Miao Ou |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Biochemistry and Biophysics Reports, Vol 5, Iss C, Pp 439-447 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2405-5808 |
| DOI: |
10.1016/j.bbrep.2015.09.022 |
| Popis: |
Environments surrounding G-rich sequences remarkably affect the conformations of these structures. A proper evaluation system mimicking the crowded environment in a cell with macromolecules should be developed to perform structural and functional studies on G-quadruplexes. In this study, the topology and stability of a G-quadruplex formed by human telomeric repeat sequences were investigated in a macromolecule-crowded environment created by polyethylene glycol 200 (PEG200), tumor cell extract, and Xenopus laevis egg extract. The interactions between small molecules and telomeric G-quadruplexes were also evaluated in the different systems. The results suggested that the actual behavior of G-quadruplex structures in cells extract is quite different from that in the PEG crowding system, and proteins or other factors in extracts might play a very important role in G-quadruplex structures. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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