Sperm DNA fragmentation, recurrent implantation failure and recurrent miscarriage
| Autor: | Carol Coughlan, Helen Clarke, Rachel Cutting, Jane Saxton, Sarah Waite, William Ledger, Tinchiu Li, Allan A Pacey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
gene regulation
Musashi Musashi-1 Musashi-2 posttranscriptional control RNA binding proteins spermatogenesis splicing testis translation cell fate cell stress importin karyopherin nucleocytoplasmic transport spermatid spermatocyte artificial insemination biomarker fertility fertilization flow cytometry infertility nanotechnology oocyte activation Postacrosomal Sheath WWI Domain Binding Protein sperm SPTRX3 thioredoxin ubiquitin ATP binding cassette transporters albumin high-density lipoprotein lipid rafts membrane fluidity membrane microdomains membrane packing oxysterols reverse cholesterol transport sterol transporters egg heat shock protein A2 molecular chaperone sperm-egg interactions dehydrogenases oxidases peroxiredoxins reactive oxygen species spermatozoa thiols thioredoxins antigen-presenting cells autoimmunity dendritic cells epididymis macrophages peripheral tolerance sperm maturation genomics male infertility proteomics sperm chromatin sperm epigenetics sperm DNA damage paternal genome offspring chemotaxis rheotaxis sperm behavior sperm motility thermotaxis apoptosis sperm capacitation conservation cryobiology endangered species male fertility blood-testis barrier ectoplasmic specialization ezrin hypogonadism Leydig cell oxidative stress Sertoli cell ultramorphology varicocele hyperthermia seminal plasma biochemical markers sperm parameters environment Hurricane Katrina New Orleans area normospermic infertile population retrospective semen analysis DNA recurrent implantation failure recurrent miscarriage Diseases of the genitourinary system. Urology RC870-923 |
| Zdroj: | Asian Journal of Andrology, Vol 17, Iss 4, Pp 681-685 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1008-682X 1745-7262 |
| DOI: | 10.4103/1008-682X.144946 |
| Popis: | Evidence is increasing that the integrity of sperm DNA may also be related to implantation failure and recurrent miscarriage (RM). To investigate this, the sperm DNA fragmentation in partners of 35 women with recurrent implantation failure (RIF) following in vitro fertilization, 16 women diagnosed with RM and seven recent fathers (control) were examined. Sperm were examined pre- and post-density centrifugation by the sperm chromatin dispersion (SCD) test and the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. There were no significant differences in the age of either partner or sperm concentration, motility or morphology between three groups. Moreover, there were no obvious differences in sperm DNA fragmentation measured by either test. However, whilst on average sperm DNA fragmentation in all groups was statistically lower in prepared sperm when measured by the SCD test, this was not seen with the results from the TUNEL assay. These results do not support the hypothesis that sperm DNA fragmentation is an important cause of RIF or RM, or that sperm DNA integrity testing has value in such patients. It also highlights significant differences between test methodologies and sperm preparation methods in interpreting the data from sperm DNA fragmentation tests. |
| Databáze: | Directory of Open Access Journals |
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