Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples

Autor: Kędrak-Jabłońska Agnieszka, Budniak Sylwia, Szczawińska Anna, Reksa Monika, Krupa Marek, Szulowski Krzysztof
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Veterinary Research, Vol 62, Iss 4, Pp 549-554 (2018)
Druh dokumentu: article
ISSN: 2450-8608
DOI: 10.2478/jvetres-2018-0075
Popis: Introduction: The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three Bacillus anthracis genes in contaminated liver and blood samples. The goals for detection were rpoB gene as a chromosomal marker, pag gene located on plasmid pXO1, and capC gene located on plasmid pXO2.
Databáze: Directory of Open Access Journals