| Autor: |
Sajoy Purathumuriyil Varghese, Janitza L Montalvo-Ortiz, John G Csernansky, Rodney I Eiger, Amy A Herrold, Maju Mathew Koola, Hongxin Dong |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Indian Journal of Psychological Medicine, Vol 37, Iss 1, Pp 36-41 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
0253-7176 |
| DOI: |
10.4103/0253-7176.150816 |
| Popis: |
Background: Early Life Stress (ELS) can profoundly influence an individual′s genotype and phenotype. Effects of ELS can manifest in the short-term, late life and even in subsequent generations. ELS activate corticotrophin releasing factor (CRF); CRF influences drug seeking and addiction. The aim of this study was to examine the effects of endogenous elevated levels of CRF on addiction. Materials and Methods: Inducible forebrain over-expression of CRF mice (tetop-CRH x CaMKII-tTA) was used for this study. Morphine (10 mg/kg) was administered every other day for 10 days or with increasing doses of morphine: 20, 40, 60, 80, 100, and 100 mg/kg. The behavioral trials including morphine sensitization, Somatic Opiate Withdrawal Symptoms (SOWS) were conducted in a single, open field, activity. After behavioral trial, animals were perfused for immunohistochemistry analysis. Results: CRF-over expressed (CRF-OE) mice showed increase in morphine sensitization and withdrawal symptoms after morphine administration compared to wild type (WT) mice. The two-way ANOVA in the morphine sensitization study showed a significant effect of treatment (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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