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Joana S Maziero,1 Velaphi C Thipe,1,2 Sizue O Rogero,1 Adriana K Cavalcante,1 Kelme C Damasceno,1 Matheus B Ormenio,1 Gisela A Martini,1 Jorge GS Batista,3 William Viveiros,4 Kavita K Katti,2 Alice Raphael Karikachery,2 DarshaKumar Dhurvas Mohandoss,5 Rashmi DarshaKumar Dhurvas,5 Mohanavelu Nappinnai,5 José R Rogero,3 Ademar B Lugão,1 Kattesh V Katti2 1Laboratório de Ecotoxicologia, Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear- IPEN/CNEN-SP, São Paulo, SP, Brazil; 2Institute of Green Nanotechnology, Department of Radiology, University of Missouri Columbia, Columbia, MO, USA; 3Laboratório de Biomateriais Poliméricos e Nanotheranóstica, Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear- IPEN/CNEN-SP, São Paulo, SP, Brazil; 4Laboratório de Ecotoxicologia, Companhia Ambiental do Estado de São Paulo (CETESB), São Paulo, SP, Brazil; 5Dhanvantari Nano Ayushadi Pvt Ltd, Chennai, Tamil Nadu, IndiaCorrespondence: Kattesh V Katti; Ademar B Lugão Email kattik@health.missouri.edu; ablugao@gmail.comIntroduction: We report, herein, in vitro, and in vivo toxicity evaluation of silver nanoparticles stabilized with gum arabic protein (AgNP-GP) in Daphnia similis, Danio rerio embryos and in Sprague Dawley rats.Purpose: The objective of this investigation was to evaluate in vitro and in vivo toxicity of silver nanoparticles stabilized with gum arabic protein (AgNP-GP), in multispecies due to the recognition that toxicity evaluations beyond a single species reflect the environmental realism. In the present study, AgNP-GP was synthesized through the reduction of silver salt using the tri-alanine-phosphine peptide (commonly referred to as “Katti Peptide”) and stabilized using gum arabic protein.Methods: In vitro cytotoxicity tests were performed according to ISO 10993– 5 protocols to assess cytotoxicity index (IC50) values. Acute ecotoxicity (EC50) studies were performed using Daphnia similis, according to the ABNT NBR 15088 protocols. In vivo toxicity also included evaluation of acute embryotoxicity using Danio rerio (zebrafish) embryos following the OECD No. 236 guidelines. We also used Sprague Dawley rats to assess the toxicity of AgNP-GP in doses from 2.5 to 10.0 mg kg− 1 body weight.Results: AgNP-GP nanoparticles were characterized through UV (405 nm), core size (20± 5 nm through TEM), hydrodynamic size (70– 80 nm), Zeta (ζ) potential (- 26 mV) using DLS and Powder X ray diffraction (PXRD) and EDS. PXRD showed pattern consistent with the Ag (1 1 1) peak. EC50 in Daphnia similis was 4.40 (3.59– 5.40) μg L− 1. In the zebrafish species, LC50 was 177 μg L− 1. Oral administration of AgNP-GP in Sprague Dawley rats for a period of 28 days revealed no adverse effects in doses of up to 10.0 mg kg− 1 b.w. in both male and female animals.Conclusion: The non-toxicity of AgNP-GP in rats offers a myriad of applications of AgNP-GP in health and hygiene for use as antibiotics, antimicrobial and antifungal agents.Keywords: nanoparticles, zebrafish, cytotoxicity, ecotoxicity, Daphnia similis, Sprague Dawley |
