| Autor: |
Eline A.J. Willemse, Ingrid S. vanMaurik, Betty M. Tijms, Femke H. Bouwman, Andreas Franke, Isabelle Hubeek, Leo Boelaarts, Jules J. Claus, Esther S.C. Korf, Rob J. vanMarum, Gerwin Roks, Niki Schoonenboom, Nicolaas Verwey, Marissa D. Zwan, Simone Wahl, Wiesje M. van derFlier, Charlotte E. Teunissen |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring, Vol 10, Iss 1, Pp 563-572 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2352-8729 |
| DOI: |
10.1016/j.dadm.2018.08.006 |
| Popis: |
Abstract Introduction We compared the automated Elecsys and manual Innotest immunoassays for cerebrospinal fluid (CSF) Alzheimer's disease biomarkers in a multicenter diagnostic setting. Methods We collected CSF samples from 137 participants in eight local memory clinics. Amyloid β(1–42) (Aβ42), total tau (t‐tau), and phosphorylated tau (p‐tau) were centrally analyzed with Innotest and Elecsys assays. Concordances between methods were assessed. Results Biomarker results strongly correlated between assays with Spearman's ρ 0.94 for Aβ42, 0.98 for t‐tau, and 0.98 for p‐tau. Using Gaussian mixture modeling, cohort‐specific cut‐points were estimated at 1092 pg/mL for Aβ42, 235 pg/mL for t‐tau, and 24 pg/mL for p‐tau. We found an excellent concordance of biomarker abnormality between assays of 97% for Aβ42 and 96% for both t‐tau and p‐tau. Discussion The high concordances between Elecsys and Innotest in this nonacademic, multicenter cohort support the use of Elecsys for CSF Alzheimer's disease diagnostics and allow conversion of results between methods. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Plný text