Elevated proton motive force is a tetracycline resistance mechanism that leads to the sensitivity to gentamicin in Edwardsiella tarda

Autor: Shao‐hua Li, Jiao Xiang, Ying‐yue Zeng, Xuan‐xian Peng, Hui Li
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Microbial Biotechnology, Vol 17, Iss 1, Pp n/a-n/a (2024)
Druh dokumentu: article
ISSN: 1751-7915
DOI: 10.1111/1751-7915.14379
Popis: Abstract Tetracycline is a commonly used human and veterinary antibiotic that is mostly discharged into environment and thereby tetracycline‐resistant bacteria are widely isolated. To combat these resistant bacteria, further understanding for tetracycline resistance mechanisms is needed. Here, GC–MS based untargeted metabolomics with biochemistry and molecular biology techniques was used to explore tetracycline resistance mechanisms of Edwardsiella tarda. Tetracycline‐resistant E. tarda (LTB4‐RTET) exhibited a globally repressed metabolism against elevated proton motive force (PMF) as the most characteristic feature. The elevated PMF contributed to the resistance, which was supported by the three results: (i) viability was decreased with increasing PMF inhibitor carbonylcyanide‐3‐chlorophenylhydrazone; (ii) survival is related to PMF regulated by pH; (iii) LTB4‐RTET were sensitive to gentamicin, an antibiotic that is dependent upon PMF to kill bacteria. Meanwhile, gentamicin‐resistant E. tarda with low PMF are sensitive to tetracycline is also demonstrated. These results together indicate that the combination of tetracycline with gentamycin will effectively kill both gentamycin and tetracycline resistant bacteria. Therefore, the present study reveals a PMF‐enhanced tetracycline resistance mechanism in LTB4‐RTET and provides an effective approach to combat resistant bacteria.
Databáze: Directory of Open Access Journals
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