Low-risk susceptibility alleles in 40 human breast cancer cell lines

Autor: Klijn Jan GM, Dehghan Abbas, Hollestelle Antoinette, Elstrodt Fons, Riaz Muhammad, Schutte Mieke
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: BMC Cancer, Vol 9, Iss 1, p 236 (2009)
Druh dokumentu: article
ISSN: 1471-2407
DOI: 10.1186/1471-2407-9-236
Popis: Abstract Background Low-risk breast cancer susceptibility alleles or SNPs confer only modest breast cancer risks ranging from just over 1.0 to1.3 fold. Yet, they are common among most populations and therefore are involved in the development of essentially all breast cancers. The mechanism by which the low-risk SNPs confer breast cancer risks is currently unclear. The breast cancer association consortium BCAC has hypothesized that the low-risk SNPs modulate expression levels of nearby located genes. Methods Genotypes of five low-risk SNPs were determined for 40 human breast cancer cell lines, by direct sequencing of PCR-amplified genomic templates. We have analyzed expression of the four genes that are located nearby the low-risk SNPs, by using real-time RT-PCR and Human Exon microarrays. Results The SNP genotypes and additional phenotypic data on the breast cancer cell lines are presented. We did not detect any effect of the SNP genotypes on expression levels of the nearby-located genes MAP3K1, FGFR2, TNRC9 and LSP1. Conclusion The SNP genotypes provide a base line for functional studies in a well-characterized cohort of 40 human breast cancer cell lines. Our expression analyses suggest that a putative disease mechanism through gene expression modulation is not operative in breast cancer cell lines.
Databáze: Directory of Open Access Journals
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