A Potential New Source of Therapeutic Agents for the Treatment of Mucocutaneous Leishmaniasis: The Essential Oil of Rhaphiodon echinus

Autor: Carlos Vinicius Barros Oliveira, Patric Anderson Gomes da Silva, Saulo Relison Tintino, Cathia Cecília Coronel, Maria Celeste Vega Gomez, Mírian Rolón, Francisco Assis Bezerra da Cunha, Maria Flaviana Bezerra Morais-Braga, Henrique Douglas Melo Coutinho, Abolghasem Siyadatpanah, Polrat Wilairatana, Jean Paul Kamdem, Luiz Marivando Barros, Antonia Eliene Duarte, Pedro Silvino Pereira
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Molecules, Vol 27, Iss 7, p 2169 (2022)
Druh dokumentu: article
ISSN: 27072169
1420-3049
DOI: 10.3390/molecules27072169
Popis: Weeds are an important source of natural products; with promising biological activity. This study investigated the anti-kinetoplastida potential (in vitro) to evaluate the cytotoxicity (in vitro) and antioxidant capacity of the essential oil of Rhaphiodon echinus (EORe), which is an infesting plant species. The essential oil was analyzed by GC/MS. The antioxidant capacity was evaluated by reduction of the DPPH radical and Fe3+ ion. The clone Trypanosoma cruzi CL-B5 was used to search for anti-epimastigote activity. Antileishmanial activity was determined using promastigotes of Leishmania braziliensis (MHOM/CW/88/UA301). NCTC 929 fibroblasts were used for the cytotoxicity test. The results showed that the main constituent of the essential oil was γ-elemene. No relevant effect was observed concerning the ability to reduce the DPPH radical; only at the concentration of 480 μg/mL did the essential oil demonstrate a high reduction of Fe3+ power. The oil was active against L. brasiliensis promastigotes; but not against the epimastigote form of T. cruzi. Cytotoxicity for mammalian cells was low at the active concentration capable of killing more than 70% of promastigote forms. The results revealed that the essential oil of R. echinus showed activity against L. brasiliensis; positioning itself as a promising agent for antileishmanial therapies.
Databáze: Directory of Open Access Journals
Nepřihlášeným uživatelům se plný text nezobrazuje