| Autor: |
Takatoshi Chujo, Koki Yoshida, Rie Takai, Osamu Uehara, Hirofumi Matsuoka, Tetsuro Morikawa, Jun Sato, Itsuo Chiba, Kenichi Matsuzaka, Yoshihiro Abiko |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Clinical and Experimental Dental Research, Vol 7, Iss 2, Pp 205-210 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2057-4347 |
| DOI: |
10.1002/cre2.355 |
| Popis: |
Abstract Objectives Epigenetic phenomena are changes in gene expression not involving the DNA sequence. DNA methylation is a major occurrence underlying epigenetic changes in human cells. Although aberrant DNA methylation is well documented in malignant lesions, limited information has been shown on the involvement of DNA methylation in oral lichen planus and oral lichenoid lesions (OLP). The present study aimed to investigate DNA methylation of E‐cadherin and p16 in OLP, and compare the findings with those in non‐inflamed gingiva (Non), radicular cyst (RC), and oral squamous cell carcinoma (SCC). Materials and methods Paraffin‐embedded surgical biopsy specimens were sliced, DNA was extracted, bisulfite treatment was applied, and methylation‐specific polymerase chain reaction was performed. Immunohistochemistry was performed to observe the relative expression patterns of these genes. Results E‐cadherin was hypermethylated in OLP (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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