| Autor: |
Hadis Musavi, Reza Iraie, Maryam Mohammadi, Zeinab Barartabar, Mohammad Yazdi, Abouzar Bagheri, Abbas Khonakdar-Tarsi |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Advanced Biomedical Research, Vol 13, Iss 1, Pp 106-106 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2277-9175 |
| DOI: |
10.4103/abr.abr_456_23 |
| Popis: |
Background: Non-alcoholic fatty liver disease is related to lipid accumulation and inflammation. Considering the role of lipin-1 and lipin-2 in fat homeostasis and inflammation, this study aimed to explore the effect of galbanic acid (Gal) and resveratrol (RSV) on alterations in the gene expression levels and protein abundance of lipin-1 and lipin-2 in HepG2 liver cells lipid-enriched with palmitate (Pal). Materials and Methods: HepG2 cells were subjected to different amounts of Gal and RSV for 24 hours in the presence of Pal to induce lipid accumulation. The RT-PCR method was employed to assess the expression of lipin-1 and lipin-2 genes, while protein levels were evaluated by western blot analysis. Lipid accumulation was determined qualitatively and semi-quantitatively using the oil-red staining technique. Results: Gal treatment increased lipin-1 and lipin-2 gene expression (P < 0.05). In contrast, the groups treated with RSV did not show a substantial variance in the expression levels of the two genes (P > 0.05). In the groups treated with Gal/RSV, the intensity of lipin-2 protein bands was higher compared to the Pal group (P > 0.01); however, the intensity of lipin-1 protein bands was not significantly different (P > 0.05). Conclusion: Gal, a coumarin compound, significantly increased the expression of lipin-1 and lipin-2 in HepG2 cells treated with Pal. Consequently, this research suggests gal as a novel strategy for regulating fat homeostasis in HepG2 cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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