THE INFLUENCE OF OVERWEIGHT AND INFLAMMATION OF PERIODONTAL TISSUES ON THE ACTIVITY OF MARKER ENZYMES OF MACROPHAGES POLARIZATION IN THE ORAL FLUID OF PRIMARY SCHOOL-AGED CHILDREN

Autor: Onyschenko A. V., Sheshukova O. V., Akimov O. Ye
Jazyk: English<br />Russian<br />Ukrainian
Rok vydání: 2020
Předmět:
Zdroj: Вісник проблем біології і медицини, Iss 4, Pp 356-360 (2020)
Druh dokumentu: article
ISSN: 2077-4214
2523-4110
DOI: 10.29254/2077-4214-2020-4-158-356-360
Popis: The aim of this research was to study the activity of inducible NO-synthase (iNOS), arginase, the concentration of nitrites and ceruloplasmin, as well as the intensity of lipid peroxidation (LPO) processes of the oral fluid in overweight children of primary school age and the signs of gum inflammation. The study included 81 students without somatic pathologies. In the presence of signs of catarrhal gingivitis in children of primary school age, the activity of gNOS in the oral fluid increases by 2.08 times as compared to healthy subjects. The cNOS activity does not change statistically significantly, and the iNOS activity increases by 2.22 times. The activity of arginases decreases by 2.21 times. In the presence of signs of catarrhal gingivitis and overweight in children of primary school age, the activity of gNOS in the oral fluid increases by 1.68 times as compared to healthy subjects. The cNOS activity does not change statistically significantly, whereas the iNOS activity increases by 1.62 times. The activity of arginases decreases by 2.21 times. The inflammatory process in the periodontal tissues against the background of overweight also reduces the activity of arginases by 2.19 times as compared to the group of patients with overweight. The concentration of nitrites, ceruloplasmin and the intensity of lipid peroxidation in the oral fluid did not statistically significantly change in all study groups. Thus, the determination of the concentrations of nitrites, ceruloplasmin, and MDA in the oral fluid in pathological processes with a low involvement of the body’s systemic response is not informative. Determination of iNOS and arginase activity in the oral fluid of patients is an informative method for assessing the body’s reactivity. The limitation of our study is the lack of an assessment of the contribution of the resident microflora of the oral cavity of patients to the studied parameters and patterns. In children of primary school age with signs of catarrhal gingivitis, regardless of the presence of overweight, there is a change in the activity of marker enzymes of macrophages polarization in the oral fluid towards an increase in the activity of the pro-inflammatory enzyme – the inducible NO-synthase. Determining the activity of marker enzymes of macrophages polarization (inducible NO-synthase and arginase) in the oral fluid is an informative test of the patient’s reactivity
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