Utility of Kumkum as a Counterstain in Histopathological Evaluation of Cervix: An Unexplored Archaic Behooveful Colourant
| Autor: | N Navya, BN Kumarguru, AS Ramaswamy, GK Swethadri, P Janakiraman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 15, Iss 4, Pp EC01-EC06 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2249-782X 0973-709X |
| DOI: | 10.7860/JCDR/2021/47668.14745 |
| Popis: | Introduction: The turmeric extract has been used as a potential alternative for eosin on routine histopathology sections. Kumkum is made from saffron flowers of Crocus sativus L. with mild use of turmeric. Aim: To evaluate the staining characteristics of kumkum solution as a counterstain and to compare the staining characteristics of kumkum solution and turmeric solution in histopathology sections of cervix tissue. Materials and Methods: It was a prospective observational study conducted at a rural tertiary care referral institute, PES Institute of Medical Sciences and Research, Kuppam, Andhra Pradesh, India, from May 2019 to July 2019. For each case, cervix tissue sections were stained with Haematoxylin and Eosin (H&E), Haematoxylin and Turmeric (H&T) and Haematoxylin and Kumkum (H&K). The staining characteristics of H&T and H&K was documented and evaluated using a scoring system by comparing with that of conventional H&E sections. The parameters were analysed for statistical significance. Chisquare test and two-sample t-test were the statistical tools used to analyse the data. All statistical calculations were done through statistical software STATA version 14.1. Results: A total of 57 cases were analysed. H&K was better than H&T with respect to contrast at low power, morphological details of cytoplasm, nucleus and acellular structures (p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|