Peritoneal sepsis caused by Escherichia coli triggers brainstem inflammation and alters the function of sympatho-respiratory control circuits

Autor: Gjinovefa Kola, Caitlyn W. Clifford, Cara K. Campanaro, Rishi R. Dhingra, Mathias Dutschmann, Frank J. Jacono, Thomas E. Dick
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Neuroinflammation, Vol 21, Iss 1, Pp 1-11 (2024)
Druh dokumentu: article
ISSN: 1742-2094
DOI: 10.1186/s12974-024-03025-7
Popis: Abstract Background Sepsis has a high mortality rate due to multiple organ failure. However, the influence of peripheral inflammation on brainstem autonomic and respiratory circuits in sepsis is poorly understood. Our working hypothesis is that peripheral inflammation affects central autonomic circuits and consequently contributes to multiorgan failure in sepsis. Methods In an Escherichia coli (E. coli)–fibrin clot model of peritonitis, we first recorded ventilatory patterns using plethysmography before and 24 h after fibrin clot implantation. To assess whether peritonitis was associated with brainstem neuro-inflammation, we measured cytokine and chemokine levels in Luminex assays. To determine the effect of E. coli peritonitis on brainstem function, we assessed sympatho-respiratory nerve activities at baseline and during brief (20 s) hypoxemic ischemia challenges using in situ-perfused brainstem preparations (PBPs) from sham or infected rats. PBPs lack peripheral organs and blood, but generate vascular tone and in vivo rhythmic activities in thoracic sympathetic (tSNA), phrenic and vagal nerves. Results Respiratory frequency was greater (p
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