Performance of TaqMan probes for the detection of sexually transmitted infections in South African women

Autor:	Nireshni Mitchev, Ravesh Singh, Nigel Garrett, Veron Ramsuran, Abraham J. Niehaus, Koleka P. Mlisana
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	sexually transmitted infections vaginal discharge syndrome molecular diagnostics validation taqman. Public aspects of medicine RA1-1270 Medicine (General) R5-920
Zdroj:	African Journal of Laboratory Medicine, Vol 10, Iss 1, Pp e1-e4 (2021)
Druh dokumentu:	article
ISSN:	2225-2002 2225-2010
DOI:	10.4102/ajlm.v10i1.1124
Popis:	Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis and Mycoplasma genitalium are the four main aetiologies of sexually transmitted infections responsible for vaginal discharge syndrome (VDS). Commercially available multiplex polymerase chain reaction (PCR) assays are expensive and generally not customisable. We evaluated a highly customisable singleplex PCR approach by testing it in parallel with the Anyplex™ II STI-7 detection assay in a cohort of South African women that presented with VDS between May 2016 and January 2017. Our multiple singleplex PCR strategy proved to be a simple, accurate, rapid, affordable and scalable option for diagnosing VDS.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/ed901c1eee314a93ba7d5cacbab28e51 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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