| Autor: |
Liu Songmei, Jiang Jie, Liu Yang, Meng Jun, Xu Shouling, Tan Yuanyuan, Li Youfa, Shu Qingyao, Huang Jianzhong |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Rice Science, Vol 26, Iss 2, Pp 88-97 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1672-6308 |
| DOI: |
10.1016/j.rsci.2019.01.002 |
| Popis: |
To explore how rice (Oryza sativa L.) can be safely produced in Cd-polluted soil, OsLCT1 and OsNramp5 mutant lines were generated by CRISPR/Cas9-mediated mutagenesis. One of OsLCT1 mutant (lct1×1) and two of OsNramp5 mutants (nramp5×7 and nramp5×9) were evaluated for grain Cd accumulation and agronomic performances. In paddy field soil containing approximately 0.9 mg/kg Cd, lct1×1 grains contained approximately 40% (0.17 mg/kg) of the Cd concentration of the wild type parental line, less than the China National Food Safety Standard (0.20 mg/kg). Both OsNramp5 mutants showed low grain Cd accumulation (< 0.06 mg/kg) in the paddy (approximately 0.9 mg/kg Cd) or in pots in soil spiked with 2 mg/kg Cd. However, only nramp5×7 showed normal growth and yield, whereas the growth of nramp5×9 was severely impaired. The study showed that lct1×1 could be used to produce rice grains safe for human consumption in lightly contaminated paddy soils and nramp5×7 used in soils contaminated by much higher levels of Cd. Keywords: cadmium, rice, OsNramp5, OsLCT1, genome-editing, heavy metal contamination, CRISPR, Cas9 |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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