| Autor: |
Ryan J. Leiphart, Hai Pham, Tyler Harvey, Taishi Komori, Tina M. Kilts, Snehal S. Shetye, Stephanie N. Weiss, Sheila M. Adams, David E. Birk, Louis J. Soslowsky, Marian F. Young |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Matrix Biology Plus, Vol 13, Iss , Pp 100099- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2590-0285 |
| DOI: |
10.1016/j.mbplus.2021.100099 |
| Popis: |
Tendon is a vital musculoskeletal tissue that is prone to degeneration. Proper tendon maintenance requires complex interactions between extracellular matrix components that remain poorly understood. Collagen VI and biglycan are two matrix molecules that localize pericellularly within tendon and are critical regulators of tissue properties. While evidence suggests that collagen VI and biglycan interact within the tendon matrix, the relationship between the two molecules and its impact on tendon function remains unknown. We sought to elucidate potential coordinate roles of collagen VI and biglycan within tendon by defining tendon properties in knockout models of collagen VI, biglycan, or both molecules. We first demonstrated co-expression and co-localization of collagen VI and biglycan within the healing tendon, providing further evidence of cooperation between the two molecules during nascent tendon matrix formation. Deficiency in collagen VI and/or biglycan led to significant reductions in collagen fibril size and tendon mechanical properties. However, collagen VI-null tendons displayed larger reductions in fibril size and mechanics than seen in biglycan-null tendons. Interestingly, knockout of both molecules resulted in similar properties to collagen VI knockout alone. These results indicate distinct and non-additive roles for collagen VI and biglycan within tendon. This work provides better understanding of regulatory interactions between two critical tendon matrix molecules. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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