Adjusting MtDNA Quantification in Whole Blood for Peripheral Blood Platelet and Leukocyte Counts.
| Autor: | Yamilee Hurtado-Roca, Marta Ledesma, Monica Gonzalez-Lazaro, Raquel Moreno-Loshuertos, Patricio Fernandez-Silva, Jose Antonio Enriquez, Martin Laclaustra |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | PLoS ONE, Vol 11, Iss 10, p e0163770 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1932-6203 49458779 |
| DOI: | 10.1371/journal.pone.0163770 |
| Popis: | Alterations of mitochondrial DNA copy number (mtDNAcn) in the blood (mitochondrial to nuclear DNA ratio) appear associated with several systemic diseases, including primary mitochondrial disorders, carcinogenesis, and hematologic diseases. Measuring mtDNAcn in DNA extracted from whole blood (WB) instead of from peripheral blood mononuclear cells or buffy coat may yield different results due to mitochondrial DNA present in platelets. The aim of this work is to quantify the contribution of platelets to mtDNAcn in whole blood [mtDNAcn(WB)] and to propose a correction formula to estimate leukocytes' mtDNAcn [mtDNAcn(L)] from mtDNAcn(WB). Blood samples from 10 healthy adults were combined with platelet-enriched plasma and saline solution to produce artificial blood preparations. Aliquots of each sample were combined with five different platelet concentrations. In 46 of these blood preparations, mtDNAcn was measured by qPCR. MtDNAcn(WB) increased 1.07 (95%CI 0.86, 1.29; p |
| Databáze: | Directory of Open Access Journals |
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