Label-Free DNA Hybridization Detection Using a Highly Sensitive Fiber Microcavity Biosensor
| Autor: | Yao Wu, Guiyu Wang, Xiujuan Yu, Yuanji Fan, Xuefeng Chen, Shengchun Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Sensors, Vol 24, Iss 1, p 278 (2024) |
| Druh dokumentu: | article |
| ISSN: | 24010278 1424-8220 |
| DOI: | 10.3390/s24010278 |
| Popis: | A novel label-free optical fiber biosensor, based on a microcavity fiber Mach–Zehnder interferometer, was developed and practically demonstrated for DNA detection. The biosensor was fabricated using offset splicing standard communication single-mode fibers (SMFs). The light path of the sensor was influenced by the liquid sample in the offset open cavity. In the experiment, a high sensitivity of −17,905 nm/RIU was achieved in the refractive index (RI) measurement. On this basis, the probe DNA (pDNA) was immobilized onto the sensor’s surface using APTES, enabling real-time monitoring of captured complementary DNA (cDNA) samples. The experimental results demonstrate that the biosensor exhibited a high sensitivity of 0.32 nm/fM and a limit of detection of 48.9 aM. Meanwhile, the sensor has highly repeatable and specific performance. This work reports an easy-to-manufacture, ultrasensitive, and label-free DNA biosensor, which has significant potential applications in medical diagnostics, bioengineering, gene identification, environmental science, and other biological fields. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|