Decellularized Antler Cancellous Bone Matrix Material Can Serve as Potential Bone Tissue Scaffold

Autor: Yusu Wang, Ying Zong, Weijia Chen, Naichao Diao, Quanmin Zhao, Chunyi Li, Boyin Jia, Miao Zhang, Jianming Li, Yan Zhao, Rui Du, Zhongmei He
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Biomolecules, Vol 14, Iss 8, p 907 (2024)
Druh dokumentu: article
ISSN: 14080907
2218-273X
DOI: 10.3390/biom14080907
Popis: Due to the limited supply of autologous bone grafts, there is a need to develop more bone matrix materials to repair bone defects. Xenograft bone is expected to be used for clinical treatment due to its exact structural similarity to natural bone and its high biocompatibility. In this study, decellularized antler cancellous bone matrix (DACB) was first prepared, and then the extent of decellularization of DACB was verified by histological staining, which demonstrated that it retained the extracellular matrix (ECM). The bioactivity of DACB was assessed using C3H10T1/2 cells, revealing that DACB enhanced cell proliferation and facilitated cell adhesion and osteogenic differentiation. When evaluated by implanting DACB into nude mice, there were no signs of necrosis or inflammation in the epidermal tissues. The bone repair effect of DACB was verified in vivo using sika deer during the antler growth period as an animal model, and the molecular mechanisms of bone repair were further evaluated by transcriptomic analysis of the regenerated tissues. Our findings suggest that the low immunogenicity of DACB enhances the production of bone extracellular matrix components, leading to effective osseointegration between bone and DACB. This study provides a new reference for solving bone defects.
Databáze: Directory of Open Access Journals
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