| Autor: |
Prawez Alam, Aftab Alam, Md. Khalid Anwer, Saleh I Alqasoumi |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 4, Pp 262-266 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2221-1691 |
| DOI: |
10.12980/APJTB.4.2014C1007 |
| Popis: |
Objective: To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits. Methods: The method was carried out in aluminum-backed silica gel 60 F254 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase. Results: A compact band was obtained for hesperidin at Rf value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.9986 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory. Conclusions: The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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